Effects of Bacillus subtilis on the Control of Powdery Mildew and Phyllospheric Bacterial Community of'Red Globe'Grapevine
In order to explore the effects of Bacillus subtilis biocontrol agents on the control of powdery mildew and phyllospheric bacterial community of grapevine,'Red Globe'grapevine was used as test material,Bacillus subtilis biocontrol agent was applied during the growing period,using a combination of microbiome and bioinformatics methods,its impact on microbial diversity and microbial community structure in grape leaf environments were investigated.The results showed that,after the treatment of Bacillus subtilis,the disease index of powdery mildew was significantly reduced.The α diversity of bacteria did not change significantly after Bacillus subtilis treatment.Non-metric multi-dimensional scale analysis showed that Bacillus subtilis treatment significantly affected the βdiversity of bacteria.Treatment with Bacillus subtilis significantly increased the relative abundance of Firmicutes and beneficial bacteria such as Bacillus and Brachybacterium genera.Meanwhile,after treated with Bacillus subtilis,the bacterial aerobic respiration I,the menaphthoquinone synthesis pathway Ⅱ,the super pathway of panquinone biosynthesis,the glycine biosynthesis of heme function decreased,and the biotin biosynthesis Ⅱ function increased significantly.Therefore,the recommended concentration of Bacillus subtilis could be used for the biological control of grapevine powdery mildew.
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