Study on Differences of Organic Acid Accumulation during Fruit Development of Table Grape
In order to explore the accumulation rule of organic acid content in table grape fruits,table grape varieties'Shine Muscat''Kyoho''Italy'and'Moldova'were used to investigate the content of organic acids,organic acid metabolism enzyme activity,and related gene expression during fruit development.The results showed that the content of organic acids gradually decreased during the fruit development process,and there were differences in total organic acid content among varieties during fruit ripening.From high to low in order,that were Kyoho(8.31 mg·g-1)＞Moldova(7.80 mg·g-1)＞Italy(7.48 mg·g-1)＞Shine Muscat(6.62 mg·g-1).Among the acids,tartaric acid was the highest content(5.17-6.19 mg·g-1),followed by malic acid(0.72-1.81 mg·g-1),and citric acid was the lowest content(0.20-0.94 mg·g-1).The activities of malic acid dehydrogenase(NAD-MDH),malate dehydrogenase(NAD-MDH),citrate synthase(CS)and expression of VvME,VvMDH genes were significantly different among the varieties.Correlation analysis showed that the content of malic acid was negatively correlated with NADP-ME activity,and positively correlated with NAD-MDH activity.Citric acid content was significantly positively correlated with CS activity.Citric acid content in'Italy'and'Moldova'grape fruits were significantly negatively correlated with mitochondrial aconitase(Mit-ACO)activity.The difference of malic acid and citric acid accumulation in four varieties was mainly caused by the differential expression of VvME,VvMDH genes,as well as differences in NADP-ME,NAD-MDH,CS and Mit-ACO enzyme activities.
万方数据
维普
