基于广泛靶向代谢组学的半遮阴处理腺枝葡萄果实差异代谢物解析
Analysis of Differential Metabolites in Vitis Adenoclada Hand.-Mass Treated with Partial Shading Based on Widely Targeted Metabolomics
盘丰平 1韦荣福 1吴代东 1成果 1张劲 1周咏梅 1谢林君 1庞丽婷1
作者信息
- 1. 广西壮族自治区农业科学院葡萄与葡萄酒研究所,广西南宁 530007
- 折叠
摘要
本试验采用腺枝葡萄'桂黑珍珠4号'(Vitis adenoclona Hand.-Mass,Guiheizhenzhu No.4)为试材,在果实开始转色至完全成熟期采用果穗半遮阴处理,测定相关理化指标,并利用广泛靶向代谢组学技术分析成熟果实代谢物的差异.结果表明,与不遮阴相比,半遮阴处理的果实粒质量下降5.49%,可溶性固形物含量提升1.65%,可滴定酸含量降低5.09%,同时加深了果实的颜色.广泛靶向代谢组分析结果表明,两处理果实中共鉴定出11类、789种代谢物,其中差异代谢物有76种,包括下调表达60种、上调表达16种,而半遮阴处理使全部糖类、大部分有机酸和花色苷类物质含量有所增加.因此果穗半遮阴处理可改善果实的酿酒品质,提升腺枝葡萄的加工潜力.
Abstract
In this staudy,'Guiheizhenzhu No.4'(Vitis adenoclona Hand.-Mass)was used as the test material.From the beginning of veraison to full maturity stage,the cluster was treated with partial shading,the related physical and chemical indexes were measured,and the differences of metabolites in mature grapes were analyzed by widely targeted metabolomics.The results showed that compared with unshaded fruit,partial shaded fruit weight decreased by 5.49%,soluble solid content increased by 1.65%,titratable acid content decreased by 5.09%,and fruit color was deepened.The results of widely targeted metabolomics analysis showed that 11 categories and 789 metabolites were identified in two treatments,of which 76 were differential metabolites(including 60 down-regulated and 16 up-regulated).However,the contents of all sugars,most organic acids and anthocyanins were increased by partial shading treatment.Therefore,the treatment of partial shading could improve the winemaking quality of fruit and improve the processing potential of V.Adenoclada fruits.
关键词
腺枝葡萄/半遮阴/广泛靶向代谢组学/类黄酮Key words
Vitis Adenoclada Hand.-Mass/partial shading/widely targeted metabolomics/flavonoids引用本文复制引用
基金项目
国家自然科学基金(32360720)
广西农业科学院科技发展基金(桂农科2021YT125)
广西农业科学院科技发展基金(桂农科2021YT126)
广西农业科学院科技发展基金(桂农科2021YT129)
出版年
2024
国家科技期刊平台
NSTL
万方数据