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葱间作对连作葡萄植株生长的转录组学分析

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为从分子水平上揭示葱缓解葡萄连作障碍的成因,以葡萄砧木'贝达'扦插苗为试材,间作四季小葱(Allium fistulosum L).采用Illumina高通量测序平台进行葡萄根尖的转录组测序,共得到38.74 Gb Clean data,葱间作后葡萄根尖共有285个基因表现出差异表达,其中上调基因为161个,下调基因为124个.KEGG分析结果表明,差异基因主要分布在植物激素信号转导途径、淀粉和蔗糖代谢途径、谷胱甘肽代谢途径、植物病原互作等.其中IAA、BAK1、SUS、GST、RPS4、PBS1基因及PIF3、ARR、WRKY52转录因子上调表达可能与间作后葡萄植株长势增强有关.研究结果为抗性基因的挖掘和筛选奠定了理论基础.
Transcriptomic Analysis of Intercropping with Scallion on the Growth of Replanting Grapevine
To investigate how scallion(Allium fistulosum L)can help overcome replant disease in grapevine at the molecular level,an experiment was conducted using'Beta'(Vitis riparia×V.labrusca)rootstock cutting seedlings as test materials and interplanted them with scallion.The transcriptome of grape root tips was sequenced by Illumina high throughput sequencing platform,and a total of 38.74Gb of Clean data were obtained.A total of 285 genes in grapevine root tips showed differential expression after intercropping with scallion,among which 161 up-regulated genes and 124 down-regulated genes.KEGG analysis results showed that the differential genes were mainly distributed in plant hormone signal transduction,starch and sucrose metabolism,and glutathione metabolism,plant-pathogen interaction,etc.Among them,the up-regulated expression of IAA,BAK1,SUS,GST,RPS4,PBS1 and PIF3,ARR,WRKY52 transcription factors may be related to the enhanced growth of grapevine seedlings after intercropping.The research results can provide a theoretical foundation for the excavating and screening of resistance genes.

grapevinereplantingscallionintercroppingtranscriptomics

李佳琪、李楠楠、李坤

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沈阳农业大学园艺学院,辽宁沈阳 110866

葡萄 连作 间作 转录组学

辽宁省教育厅项目

LJKZ0636

2024

中外葡萄与葡萄酒
中国酿酒工业协会 山东省酿酒葡萄科学研究所

中外葡萄与葡萄酒

北大核心
影响因子:0.46
ISSN:1004-7360
年,卷(期):2024.(4)
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