Transcriptomic Analysis of Intercropping with Scallion on the Growth of Replanting Grapevine
To investigate how scallion(Allium fistulosum L)can help overcome replant disease in grapevine at the molecular level,an experiment was conducted using'Beta'(Vitis riparia×V.labrusca)rootstock cutting seedlings as test materials and interplanted them with scallion.The transcriptome of grape root tips was sequenced by Illumina high throughput sequencing platform,and a total of 38.74Gb of Clean data were obtained.A total of 285 genes in grapevine root tips showed differential expression after intercropping with scallion,among which 161 up-regulated genes and 124 down-regulated genes.KEGG analysis results showed that the differential genes were mainly distributed in plant hormone signal transduction,starch and sucrose metabolism,and glutathione metabolism,plant-pathogen interaction,etc.Among them,the up-regulated expression of IAA,BAK1,SUS,GST,RPS4,PBS1 and PIF3,ARR,WRKY52 transcription factors may be related to the enhanced growth of grapevine seedlings after intercropping.The research results can provide a theoretical foundation for the excavating and screening of resistance genes.
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