首页|芽孢杆菌与化学杀菌剂复配防治葡萄灰霉病的研究

芽孢杆菌与化学杀菌剂复配防治葡萄灰霉病的研究

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  • 国家科技期刊平台
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为了减少防治葡萄灰霉病化学农药的使用，提高生防芽孢杆菌防治灰霉病的作用效果，本研究采用平板对峙法和生长速率法筛选出防治葡萄灰霉病的有效化学杀菌剂和生防芽孢杆菌，并对二者进行复配，通过室内离体接种和盆栽试验明确复配药剂对灰霉病的防治效果。结果表明，化学杀菌剂异菌脲和贝莱斯芽孢杆菌GSBZ09对灰霉病菌的抑制效果较好，异菌脲的EC50值最低，为6。46 mg·L-1;贝莱斯芽孢杆菌GSBZ09的拮抗作用最强，且异菌脲对贝莱斯芽孢杆菌GSBZ09的生长无影响。本研究中异菌脲和贝莱斯芽孢杆菌GSBZ09的复配体积比为3∶1时，防治效果最好，对葡萄灰霉病离体果实防治效果高达92。93%;对盆栽植株的防效为80。63%，均优于异菌脲单剂和贝莱斯芽孢杆菌GSBZ09单剂的防治效果。
Research on Combination of Bacillus and Fungicide to Control Gray Mold of Grapevine
In order to reduce the use of chemical pesticides to control gray mold of grapevine and improve the effect of biocontrol Bacillus on Botrytis cinerea,in this study,effective chemical agents and biocontrol Bacillus for gray mold of grapevine were screened by plate confrontations and growth rate methods,and the two were combined,and the control effect of the combined agents on gray mold of grapevine was confirmed through indoor inoculation and pot experiment.The results showed that the chemical iprodione and Bacillus velezensis GSBZ09 had the best inhibition effect on Botrytis cinerea,and the EC50 value of iprodione was the lowest(6.46 mg·L-1).The antagonistic effect of Bacillus velezensis GSBZ09 was the strongest,and iprodione had no effect on the growth of Bacillus velezensis GSBZ09.When the combined volume ratio of iprodione and Bacillus velezensis GSBZ09 was 3∶1,the control effect of indoor and pot experiment was as high as 92.93% and 80.63%,respectively,which was superior to the control effect of isomylurea single and Bacillus velezensis GSBZ09 single.

Gray mold of grapevinefungicidebiocontrol Bacilluscompoundprevention

尹向田、迟军利、韩星、李廷刚、刘其宝、魏彦锋、杨国伟、袁丽芳

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山东省葡萄研究院,山东济南 250100

烟台市蓬莱区葡萄与葡萄酒产业发展服务中心,山东蓬莱 265600

葡萄灰霉病 杀菌剂 生防芽孢杆菌 复配 防治

山东省自然科学基金山东省农业科学院创新工程山东省农业科学院创新工程山东省农业科学院创新工程山东省农业科学院创新工程山东省葡萄研究院引导基金山东省葡萄研究院引导基金山东省葡萄研究院引导基金

ZR2021QC131CXGC2023A41CXGC2023A47CXGC2022E15CXGC2024D16SDAG2021B10SDAG2021B06SDAG2021B02

2024

10.13414/j.cnki.zwpp.2024.04.015

中外葡萄与葡萄酒
中国酿酒工业协会　山东省酿酒葡萄科学研究所

中外葡萄与葡萄酒

北大核心
影响因子：0.46
ISSN：1004-7360
年,卷(期)：2024.(4)
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