Molecular Identification of Two New Self-incompatible Alleles(S-alleles)in Chinese Pear(Pyrus bretschneideri)
Self-incompatibility(SI) is an important in traspecific reproductive barrier in flowering plants.To identify the S-alleles of Chinese pear species(Pyrus pyrifolia,P.bretschneideri,P.ussuriensis and P.sinkiangenis etc.),S-RNase-specific PCR amplification,sequence analyses and field pollination tests were perbretschneideri as materials.Two new S-RNase genes were identified from the two cultivars.They were 1122 bp and 1058 bp in length,and designated as S37-RNase (GenBank accession no.DQ839238)and S38-RNase (GenBank accession no.DQ839239).By comparison of their deduced amino acid sequences with those of S1-to S36-alleles of Oriental pear,it can be seen that both the two new S-alleles had their conserved regions C1 and C2,but their hypervariable regions(HV)were quite different from those of the others.S37 showed a higher similarity(96%)to S38 in the amino acid sequences deduced from them,whereas both of them displayed the highest similarity(98%)to S15 and the lowest(63%)to S32.The two S-alleles had introns of 786 bp and 723 bp,respectively,similar in size to that of S15(777 bp).Finally,ously determined as S34S37 and S15S38,respectively.
NETL
NSTL
万方数据
维普
