中国梨2个自交不亲和新等位基因(S等位基因)的分子鉴定
Molecular Identification of Two New Self-incompatible Alleles(S-alleles)in Chinese Pear(Pyrus bretschneideri)
谭晓风 1张琳 1乌云塔娜 1袁德义 1曹玉芬 2姜傲芳 1梁文杰 1曾燕玲1
作者信息
- 1. 中南林业科技大学经济林育种与栽培国家林业局重点实验室,长沙,410004
- 2. 中国农业科学院果树研究所,辽宁兴城,125100
- 折叠
摘要
自交不亲和是显花植物的一种重要生殖生理现象,为探明中国梨的自交不亲和特性,对'锦香'(Pyrus bretschneideri cv.Jinxiang)和'鹅酥'(Pyrusbretschneideri cv.Esu)2个中国梨品种进行了基因组PCR特异扩增、S基因序列分析及田间杂交授粉试验.结果确定它们各含1个新S-RNA酶基因,分别命名为S37-和S38-RNase,GenBank序列号为DQ839238和DQ839239.生物信息学分析结果表明,S37-和S38-RNA酶的推导氨基酸序列与S1-至S36-RNA酶36个梨s基因具有相同的、高度保守的C1和C2区,但其高变区与S1-至S36-RNA酶差异较大,其中与S15的差异最小,只有3个氨基酸不同.在推导的氨基酸水平上,S37与S38有96%的序列相似性,但两者与S15的相似性更高,皆为98%,与S32的相似性最低,都只有63%;S37和S38的内含子较大,分别为786 bp和723 bp,与S15的777 bp大小接近.最后,经分析验证确定'锦香'和'鹅酥'的S基因型分别为S34S37和S15S38.
Abstract
Self-incompatibility(SI) is an important in traspecific reproductive barrier in flowering plants.To identify the S-alleles of Chinese pear species(Pyrus pyrifolia,P.bretschneideri,P.ussuriensis and P.sinkiangenis etc.),S-RNase-specific PCR amplification,sequence analyses and field pollination tests were perbretschneideri as materials.Two new S-RNase genes were identified from the two cultivars.They were 1122 bp and 1058 bp in length,and designated as S37-RNase (GenBank accession no.DQ839238)and S38-RNase (GenBank accession no.DQ839239).By comparison of their deduced amino acid sequences with those of S1-to S36-alleles of Oriental pear,it can be seen that both the two new S-alleles had their conserved regions C1 and C2,but their hypervariable regions(HV)were quite different from those of the others.S37 showed a higher similarity(96%)to S38 in the amino acid sequences deduced from them,whereas both of them displayed the highest similarity(98%)to S15 and the lowest(63%)to S32.The two S-alleles had introns of 786 bp and 723 bp,respectively,similar in size to that of S15(777 bp).Finally,ously determined as S34S37 and S15S38,respectively.
关键词
自交不亲和/聚合酶链式反应/序列分析/S基因型/S-RNA酶/S等位基因Key words
self-incompatibility(SI)/PCR/sequence analysis/S-genotypes/S-RNase/S-allele引用本文复制引用
基金项目
林业部科研项目(2006.12)
Key Project of State Forestry Administration(2006-12)
出版年
2007
NETL
NSTL
维普
万方数据