Salt and Drought Tolerance of Transgenic Salvia miltiorrhiza Bunge with the TaLEA1 Gene
TaLEA1,a gene encoding a late-embryogenesis-abundant protein,was cloned from wheat and was transformed into Salvia Miltiorrhiza Bunge by Agrobacterium-mediated leaf-disk transformation method.Seven transgenic lines were obtained after kanamycin(50 mg/L) screening.Six positive lines were obtained by PCR amplification,and after four additional generations,one stable line was obtained by Southern hybridization.Transgenic plants had better growth states than control plants on mediums containing 1% NaC1 and 8% PEG6000,which demonstrated that TaLEA1 played an important role in increasing the salt and drought tolerance of S.miltiorrhiza.
Salvia miltiorrhiza BungeAgrobacterium tumefaciensTaLEA1 genesalt stressdrought stress
NETL
NSTL
万方数据
维普
