Changes in α-L-Arabinofuranosidase Activity in Peel and Pulp of Banana (Musa sp.)Fruits during Ripening and Softening
Arabinose is one of the most dynamic cell wall glycosyl residues released during fruit ripening.α-Larabinofuranosidase(α-Arab)are major glycosidases that may remove arabinose units from fruit cell wall polysaccharides.To find out whether α-Arab plays importam roles in banana fruit softening,the enzyme activities in peel and pulp,fruit firmness,respiration rate and ethylene release rate were assayed dunng banana softening.The results showed that α-Arab activities in banana pulp and peel increased slightly at the beginning of storage and reached their maxima when the fruit firmness decreased drastically.α-Arab activity increased by more than ten folds in both pulp and peel during ripening and α-Arab activities were higher in pulp than in peel.Treatment of banana fruits with ethylene absorbent postponed the time of reaching of its maxima of respiration and ethylene,enhanced the firmness of pup and decreased α-Arab activity in the peel and pulp.These results suggest that α-Arab induced the decrease of fruit firmness and played an important role in banana fruit softening,and its activity is regulated by ethylene.
banana fruitripening and softeningα-L-arabinofuranosidase activity