一氧化氮供体硝普钠浸种缓解盐胁迫对小麦种子萌发的抑制作用
Priming of Seeds with Nitric Oxide Donor Sodium Nitroprusside (SNP) Alleviates the Inhibition on Wheat Seed Germination by Salt Stress
段培 1丁烽 1王芳 1王宝山1
作者信息
- 1. 山东师范大学生命科学学院,济南,250014
- 折叠
摘要
以小麦品种'德抗961'为材料,用NO供体硝普钠(SNP)浸种研究外源NO对盐胁迫下小麦种子萌发的影响.结果表明:0.06 mmol/L的SNP浸种24 h后对盐胁迫下小麦种子发芽率、发芽指数、活力指数和吸胀速率的下调都有显著缓解作用;SNP浸种对盐胁迫下α-淀粉酶的活性无明显影响,但能显著提高盐胁迫下β-淀粉酶的活性;进一步研究表明,SNP浸种预处理对盐胁迫下的α-淀粉酶同工酶变浅的条带有所恢复(尤其是条带3),同时使盐胁迫下变浅的β-淀粉酶同工酶的条带有明显的恢复(尤其是d、e、f、g).并且SNP能显著降低盐胁迫下小麦地上部分和根中的Na+含量,提高其K+含量,从而使K+/Na+显著提高.以上结果表明:SNP浸种预处理提高盐胁迫下小麦种子的萌发,主要是通过提高β-淀粉酶的活性来实现的.
Abstract
The effect of SNP, an NO donor, on seed gerunder salt stress was studied. The results showed that priming of seeds with 0.06 mmol/L SNP for 24 h markedly alleviated the decrease of the germination percentage, germination index, vigor index and imbibition rate of wheat seeds under salt stress. SNP significantly alleviated the decrease of the β-amylase activity but almost did not affect the α-amylase activity of wheat seeds under salt stress. SNP slightly increased the α-amylase isoenzymes (especially isoenzyme 3) and significantly increased the β-amylase isoenzymes (especially isoenzyme d, e, f and g). SNP pretreatment decreased Na+ content, but increased the K+ content, resulting in a mark increase of K+/Na+ ratio of wheat seedlings under salt stress. These results suggested that NO is involved in promoting wheat seed germination under salt stress by increasing the β-amylase activity.
关键词
一氧化氮(NO)/盐胁迫/小麦/萌发/离子含量Key words
nitric oxide(NO)/salt stress/wheat/germination/ion content引用本文复制引用
基金项目
国家自然科学基金(30270793)
国家自然科学基金(30670177)
National Research Foundation for Doctoral Program of High Education of China(20050445003)
出版年
2007
NETL
NSTL
维普
万方数据