Priming of Seeds with Nitric Oxide Donor Sodium Nitroprusside (SNP) Alleviates the Inhibition on Wheat Seed Germination by Salt Stress
The effect of SNP, an NO donor, on seed gerunder salt stress was studied. The results showed that priming of seeds with 0.06 mmol/L SNP for 24 h markedly alleviated the decrease of the germination percentage, germination index, vigor index and imbibition rate of wheat seeds under salt stress. SNP significantly alleviated the decrease of the β-amylase activity but almost did not affect the α-amylase activity of wheat seeds under salt stress. SNP slightly increased the α-amylase isoenzymes (especially isoenzyme 3) and significantly increased the β-amylase isoenzymes (especially isoenzyme d, e, f and g). SNP pretreatment decreased Na+ content, but increased the K+ content, resulting in a mark increase of K+/Na+ ratio of wheat seedlings under salt stress. These results suggested that NO is involved in promoting wheat seed germination under salt stress by increasing the β-amylase activity.