镉诱导的菊芋幼苗膜脂过氧化和抗氧化酶活性及过氧化物酶同工酶的改变
Cadmium-induced Membrane Lipid Peroxidation and Changes in Antioxidant Enzyme Activities and Peroxidase Isoforms in Jerusalem Artichoke Seedlings
陶毅明 1陈燕珍 1梁杨琳 1徐美燕 1徐祥明1
作者信息
- 1. 广西师范大学生命科学学院,桂林,541004
- 折叠
摘要
用含有不同浓度(0～400 μmol/L)Cd(NO3)2的Hoagland营养液处理砂培的菊芋.处理50 d后,测定植物体内镉积累量以及过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性,并对POD同工酶进行电泳分析.发现在Cd 50～100 μmol/L浓度内,随着镉浓度的升高,菊芋根和叶中镉的积累量显著增加,而随后积累量的增加有所减少.根和叶中MDA含量显著上升,说明镉引起了膜脂过氧化.0～100 μmol/L Cd处理,根和叶中POD活性随Cd浓度增加而增强,而在200～400 μmol/L Cd处理下有所减弱.根和叶SOD活性在50～200 μmol/L Cd处理下随Cd浓度增加而增强,而在400μmol/L Cd处理下SOD活性明显受到抑制.根和叶CAT活性随Cd浓度升高而增强.电泳结果显示,POD同工酶变化明显,镉诱导出一条新酶带LP10.菊芋POD同工酶可以作为镉污染的土壤的生物指示剂.
Abstract
Jerusalem artichoke (Helianthus tuberosus L.)seedlings cultured in sandy media were treated with Hoagland nutrition solution with different concentrations of Cd(NO3)2 from 0 to 400 μmol/L. After 50 days' treatment, Cd accumulation, activities of peroxidase (POD, EC 1.11.1.7), superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6) were measured and electrophoretograms of POD isoenzymes were analyzed. The accumulation of Cd in seedlings increased from Cd 50-100 μmol/L, after which further increases in Cd concentration resulted in only small increases in accumulation of Cd in seedlings. MDA content was markedly higher than control values indicating the enhanced membrane lipid peroxidation in roots and leaves. POD activities in leaf and root extracts increased with an increase of Cd concentration from 0 to 50 and 100 μmol/L and then decreased with further increases to 200 and 400 μmol/L. Under moderate Cd level of 50-200 μmol/L,SOD activities in leaf and root extracts increased whereas with a higher Cd level of 400 μmol/L marked inhibitions in enzyme activities were observed. With increase in Cd concentration marked elevations in CAT activities in leaves and roots were observed. Results of electrophoresis show that the alteration of POD isoenzyme was noticeable to Cd and an additional POD isoenzyme LP10 appeared. It is suggested that POD isoenzyme of Jerusalem artichoke seedlings could be used as bioindicator for soil contamination by Cd.
关键词
抗氧化酶/镉/菊芋/膜脂过氧化Key words
antioxidative enzymes/cadmium/Jerusalem artichoke/membrane lipid peroxidation引用本文复制引用
基金项目
广西博士学位授予点学科建设专项经费资助项目(XKY2006ZD02)
出版年
2007
NETL
NSTL
维普
万方数据