利用RNA干扰技术获得晚抽薹开花转基因大白菜
Late-Bolting Transgenic Chinese Cabbage Obtained by RNA Interference Technique
夏广清 1朱俊义 1何启伟 2赵双宜 3王翠花2
作者信息
- 1. 通化师范学院生物系,吉林通化,134001
- 2. 山东省农业科学院蔬菜研究所,济南,250100
- 3. 山东大学生命科学学院,济南,250100
- 折叠
摘要
LEAFY基因在植物的成花转变中起重要作用.在拟南芥中,降低LEAFY基因的表达使开花延迟,并且增加分枝.利用RNAi技术降低大白菜LEAFY基因表达,获得晚抽薹开花的转基因大白菜.开花时转基因大白菜的株高明显低于对照,而叶片数、叶面积和侧枝数则高于对照;转基因植株的LEAFY基因表达明显降低,导致大白菜抽薹开花延迟.因此可以通过降低LEAFY基因表达来延迟需低温春化作用长日植物开花.也为利用生物技术获得晚抽薹转基因大白菜提供了理论依据.
Abstract
LEAFY (LFY) gene plays an important role in determining plant flowering mainly by controlling the timing of phase transition.Constitutive under-expression of LFY in Arabidopsis resulted in the formation of a lateflowering and highly branching phenotype.In this paper,an RNAi approach was used in down-regulated LFY gene expression to delay Chinese cabbage(Brassica rapa L.ssp.pekinensis)bolting and flowering.The results show that transgenic plant has a later transition to the reproductive phase,and the transgenic plants have more branches,more leaves,but a lower height.Results of RQRT-PCR analysis show that LFY gene expression was greatly reduced in transgenic plants.These results suggest that inhibiting LFY gene expression by RNA interference can delay bolting in a cold-sensitive long-day (LD)condition-Late flowering of Chinese cabbage can be used as a good genetic resource for the breeding latebolting Chinese cabbage
关键词
大白菜/LEAFY基因/RNAi/抽薹开花Key words
Chinese cabbage/LEAFY gene/RNA interference/bolting and flowering引用本文复制引用
出版年
2007
NETL
NSTL
维普
万方数据