Late-Bolting Transgenic Chinese Cabbage Obtained by RNA Interference Technique
LEAFY (LFY) gene plays an important role in determining plant flowering mainly by controlling the timing of phase transition.Constitutive under-expression of LFY in Arabidopsis resulted in the formation of a lateflowering and highly branching phenotype.In this paper,an RNAi approach was used in down-regulated LFY gene expression to delay Chinese cabbage(Brassica rapa L.ssp.pekinensis)bolting and flowering.The results show that transgenic plant has a later transition to the reproductive phase,and the transgenic plants have more branches,more leaves,but a lower height.Results of RQRT-PCR analysis show that LFY gene expression was greatly reduced in transgenic plants.These results suggest that inhibiting LFY gene expression by RNA interference can delay bolting in a cold-sensitive long-day (LD)condition-Late flowering of Chinese cabbage can be used as a good genetic resource for the breeding latebolting Chinese cabbage
Chinese cabbageLEAFY geneRNA interferencebolting and flowering
NETL
NSTL
万方数据
维普
