低能离子束促进小麦copia-反转录转座子的转录与转座
Low-Energy Ion Beam Promotes the Transcription and Transposition of the Copia-retrotransposons in Wheat(Triticum aestivum L.)
押辉远 1谷运红 2焦浈 2王卫东 2秦广雍 2霍裕平2
作者信息
- 1. 郑州大学河南省离子束生物工程重点实验室,郑州,450052;洛阳师范学院生命科学系,洛阳,471022
- 2. 郑州大学河南省离子束生物工程重点实验室,郑州,450052
- 折叠
摘要
LTR-反转录转座子是真核基因组重要的组成部分,能通过RNA中间体完成在基因组上的转座.小麦种子受低能氮离子束注入后能促进其反转录转座子的转录,经不同剂量的氮离子束注入的种子发芽24 h和48 h后,其中的copia-反转录转座子的转录都有不同程度的提高,最高可达到对照的40倍.用基于反转录转座子扩增多态性和反转录转座子微卫星扩增多态性2种分子标记技术扩增经低能氮离子注入后的小麦DNA,指纹图谱显示出了一定的多态性,这说明低能氮离子束激活了小麦中反转录转座子的转座活性.转座子转录活性的增强可调节其邻近基因的表达和mRNA的拼接,反转录转座子插入到基因组上新的位点后,使基因组上的基因发生了重排,这种重排可能表现为植物表型的改变.因此,推测反转录转座子的转录活性提高和转座激活是产生低能离子束注入生物效应的重要原因之一.
Abstract
LTR-retrotransposons are genetic elements having the direct long terminal repeats(LTRs).It can move via an RNA intermediate within genomes and is an important fraction of eukaryote genomes.Low-energy N+ion beam promoted the transcription of the copiaretransposons in those wheat(cv.'Zhoumai 16'),which were radiated and allowed to grow for 24 h and 48 h from the planting.Relative expression ratio of the copiaretransposons was elevated in different degrees(with a max 40 fold)in wheat plants treated with different doses of N+beam.comparing to that in the controls.The molecule markers of the IRAP and REMAP to the DNA iso lated from the 14-dleaves of wheat plants treated with the low-energy N+beam showed that the transposition of some copia-retransposons was re-activated.The enhanced transcription of the copia-retransposons in wheat could weaken or enhance the expression of their nearby genes.The transposition of the retrotransposon in genome can change the primary structure of the functional DNA fragments of chromosomes,and it can also be visualized as the appearance of a new phenotype of plants.In the mid 1980s,the biological effects of low-energy ion beam were recognized and demonstrated experimentally.Hence,it suggests that the enhanced transcription and the re-activated transposition of the retrotransposons are partially attributed to the biological effect of low-energy ion beam.
关键词
低能离子束/copia-反转录转座子/转录/转座/小麦Key words
low-energy ion beam/copia-retrotransposons/transcription/transposition/wheat引用本文复制引用
出版年
2007
NETL
NSTL
维普
万方数据