Cloning and Expression of Resistance Gene Analogs(RGAs)from Wild Banana Resistant to Banana Fusarium Wilt
Wild banana species are essential natural gene pools for banana improvement.In this study,six RGAs about 500 bp were obtained from 1eaves of Musa acuminata,a wild banana shown to be resistant to ba nana Fusarium wilt race 4,by PCR amplification with degenerate primers designed according to the conserved NBS motif and serine/threonine kinase domain of plant resistance(R)genes.Among these RGAs,the deduced amino acids of WNB1 and WNB2 contain NB-ARC do main and WNB1 can be translated into polypeptide uninterrupted by stop codons.The deduced amino acids of other four RGAs (WST1,WST2,WST3 and WST4)all contain the serine/threonine kinase domain and WST3encodes a polypeptide homologous to that of bacterial blight resistance gene Xa21 of rice.At difierent time at ter inoculation with Fusarium oxysporum f.sp.cubense (FOC)race 4,the transcript pattems of WNB1 and WST3 was enhanced,which implied that the expression of WNB1 and WST3 may be related to the resistance of banana to Fusarium wilt.
Musa acuminataFusarium wiltresistance gene analogs(RGAs)cloningexpression
NETL
NSTL
万方数据
维普
