来自中国云南野生稻的抗稻瘟病遗传资源
A Rice Blast-resistance Genetic Resource from Wild Rice in Yunnan,China
杨明挚 1程在全 2陈善娜 1钱君 2徐玲玲 1黄兴奇2
作者信息
- 1. 云南大学生命科学学院,昆明,650091
- 2. 云南省农业科学院生物技术研究所,昆明,650223
- 折叠
摘要
与Pi-ta'等位基因相比,含有Pi-ta+等位基因的栽培稻具有抗稻瘟病特性.本研究用基因序列分析的方法检测了来自云南的不同栽培稻品种以及不同类型和来源的普通野生稻种和非洲长雄蕊野生稻种中的Pi-ta基因,发现Pi-ta基因在稻属植物中高度保守,但Pi-ta+等位基因的存在极其稀有.在所检测的栽培稻和野生稻中仅有来源于云南景洪的直立型普通野生稻中含有Pi-ta+等位基因.而与Pi-ta基因相比,另一个水稻抗稻瘟病基因Pib,经部分同源序列克隆及测序发现该基因在不同野生稻中的变异较大.在所克隆的不同野生稻Pib基因同源序列中,也只有来源于直立型普通野生稻的序列能按该基因的开放阅读框进行正常翻译.对不同类型普通野生稻的抗稻瘟病能力的初步鉴定结果表明,直立型普通野生稻对供试的本地稻瘟病生理小种具有较强抗性,其抗性可能源于所含的Pi-ta+等位基因及可能有功能的Pib基因.由于普通野生稻与栽培稻同属AA基因组型,因此,云南直立型普通野生稻可通过杂交育种或基因工程途径用于栽培稻的抗稻瘟病性能改良.
Abstract
Compared to Pi-ta+ alleles,Pi-ta+ alleles can cause blast resistance response.In this work,Pi-ta gene in multiple rice materials,including local rice cultivars,different types of O.rufipogon and O.longistaminata was detected by molecular cloning and sequence analysis.Results indicated that Pi-ta+ alleles were rare alleles,be O.rufipogon(ETOR)from Jinghong county in Yunnan province contains a Pi-ta+ allele.Another rice blast resistance gene,Pib,confers resistance to the Japanese strain of M.grisea.was also confirmed to be functional in this type of O.rufipogon.The results of pathogen inoculation test show that ETOR is more strongly resistant to the tested blast pathogen races than other types of O.rufipogon.The resistance of ETOR may at least partially depend upon the functioning of Pi-ta and Pib gene.As O.rufipogon has the same type of genome with the cultivated rice(D.sativa),Pi-ta+ and Pib gene in Erect type of O.rufipogon can be used to improve the tolerance of cultivated rice to blast,either by traditional hybridization or by genetic engineering.
关键词
直立型普通野生稻/Pi-ta+等位基因/Pib基因同源序列/抗稻瘟病遗传资源Key words
'Erect'type of O.rufipogon (ETOR)/Pi-ta+allele/Pib gene homologues/rice blast-resistance genetic resource引用本文复制引用
基金项目
国家自然科学基金(30069003)
云南省自然科学基金(200600007Q.2004C0010Z)
出版年
2007
NETL
NSTL
维普
万方数据