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甘薯EST资源的SSR信息分析

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原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
从NCBI公共数据库下载获得22 371条甘薯EST序列,去除低质量的和冗余的序列后,得到总长为5.09×10~3 kb的9204条唯一序列.从这些序列中搜索到总共436个SSR位点,平均相距11.68 kb出现一个SSR.这些SSR的出现频率和平均长度分别为4.4%和24.28 bp.在2～6 bp的重复基元中,六核苷酸重复基元出现频率最高(30.96%),其次是三核苷酸重复基元(29.59%)和二核苷酸重复基元(24.54%).出现最多的重复基元是AG/CT(16.28%),其次是AAG/CTT(11.01%).
Analysis of SSR Information in EST Resource of Sweet Potato [Ipomoea batatas (L.) Lam]
In this study, 22 371 ESTs of sweet potato (Ipomoea batatas) in the database of NCBI were downloaded and some redundants or with low qualities were removed, finally 9 204 unique sequences with total length about 5.09?0~3 kb were obtained. In total, 436 SSRs (4.4%) containing unique sequences were identified. The overall average length of SSRs was 24.28 bp. This was equivalent to 1 SSR per 11.68 kb EST sequence. About 30.96% of the SSRs was hexanucleotides, 29.59% was trinucleotides, 24.54% was dinucleotides, and the remaining 14.91% consisted of tetra- and pentanucleotides. Among the identified SSRs, AG/CT was the most frequent (16.28%) followed by AAG/CTT at 11.01%.

sweet potatoEST-SSRmotifs

黄立飞、房伯平、陈景益、张雄坚、罗忠霞

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广东省农业科学院作物研究所,广州,510640

甘薯 EST-SSR 基元

国家高技术研究发展计划(863计划)国家科技基础条件平台建设项目广东省自然科学基金广东省科技计划项目

2006AA1001072005DKA210020581510640010000332008B020200003

2009

植物生理学报
中国植物生理学会，中国科学院上海生命科学研究院植物生理生态研究所

植物生理学报

CSTPCDCSCD北大核心
影响因子：1.532
ISSN：2095-1108
年,卷(期)：2009.45(1)
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