Reorganization and Structure Analysis of MBL II Gene
Mabinlin II is the unique plant sweet protein from Capparis masaikai which is only found in China but no sweetness gene expression products in vitro was reported so far. MBL II gene was constructed into plant expression vector, and the recombinant MBL II was transformed into Arabidopsis. Gene expressions in transgenic Arabidopsis were detected by RT-PCR method. MBL II gene and the recombinant MBL II genes were analyzed and predicted by bioinformatics. The transgenic Arabidopsis plants were tested no sweet. But the RT-PCR results showed that MBL II gene and the restructure MBL II genes were expressed in the transgenic Arabidopsis. Bioinformatics analysis also showed that the recombinant mabinlin II proteins maybe a sweet protein.
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