Somatic Embryogenesis and Plant Regeneration of Caragana fruticosa from Cotyledons of Its Young Zygotic Embryos
Somatic embryogenesis and plant regeneration was conducted by taking cotyledons of young zygotic embryos as explants. Somatic embryos were obtained on MS medium supplemented with 500 mg·L-1 hydrolyzed casein, 30 g·L-1 sugar, 8 g·L-1 agar and different concentrations of BA and NAA or 2,4-D. The best combinations of plant growth regulators (PGRs) were 5 mg·L-1 NAA+5 mg·L-1 BA and 5 mg·L-1 2,4-D+1 mg·L-1 BA with a inductivity of 14％ and 10％ and maximum somatic embryos per explant of 4.5 and 10.5 respectively.The somatic embryos maturated on MS medium without PGRs, germinated on MS supplemented with NAA 0.01 mg·L-1, sugar 20 g·L-1, agar 6 g·L-1 at the germination rate of 58.94％. Germinated somatic embryos were able to transform plantlets on MS medium with a transformation rate of 87％. Acclimated plantlets grew normally with 40％ transplanting survival rate in growing medium in composition of 50％ peat moss, 40％ vermiculite and 10％ polite.
NETL
NSTL
万方数据
维普
