Different inbred lines of pak-choi (Brassica campestris ssp. Chinensis Makino) were used to study the contents of carbohydrate, soluble protein and free amino acids of leaves at six developmental stages. The contents of total soluble sugar and sucrose increased during the flower bud differentiation, and then decreased, but increased while bolting. The content of reducing sugar increased at the middle differentiation stage. Compared to the three inbred lines, the contents of sugars in line 08-1P-89 with late bolting were the highest during the flower bud differentiation. The tendency was similar among the contents of soluble protein, free amino acids and total soluble sugar during the whole process, except the free amino acids content decreased significantly while bolting, and the contents of soluble protein and free amino acids in late bolting inbred line were the highest. Carbon-nitrogen ration showed an increasing trend at flower bud differentiation stage and bolting stage.