G-Protein Acts Upstream of H2O2 in Mediating H2S-Induced Stomatal Closure in Arabidopsis thaliana
Hydrogen sulfide (H2S) induces stomatal closure in Arabidopsis thaliana, hydrogen peroxide (H2O2) plays an important part in this procedure. In this paper, Arabidopsis wide type and its a subunit deficient mutants (Atgpal-3 and Atgpal-4) as well as β subunit deficient mutants (Atagb1-1 and Atagb1-2) were used as materials, the effects of G-protein on H2S-induced stomatal closure and the relationship between G-protein and H2O2 were investigated. The results showed that H2S induced stomatal closure as well as the expression of G-protein a subunit (GPA) and β subunit (AGB) gens in leaves of Arabidopsis wild type, but had no significant effects on stomatal movement in Atgpa1-3, Atgpa1-4, Atagb1-1 and Atagb1-2 mutants. GPA activator cholera toxin (CTX) improved H2S-induced stomatal closure, but its inhibitors pertussis toxin (PTX) showed the opposite effect. These results manifested that G-protein was an signaling intermediate in H2S-induced stomatal closure. H2S induced increase in H2O2 content in leaves and guard cells of A. thaliana wide type, but not in Atgpa1-3, Atgpa1-4, Atagb1-1 and Atagb1-2 mutants. CTX enhanced H2O2 content in leaves and guard cells of Arabidopsis, but its inhibitor PTX was opposite. From these data it could be deduced that G-protein acts upstream of H2O2 in mediating H2S-induce stomatal closure in Arabidopsis.
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万方数据
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