Establishment of High Frequency and Direct Regeneration System from Leaf of ‘Hayward' Kiwifruit [Actinidia deliciosa (A.Chev.) C.F.Liang et A.R.Ferguson]
Leaves excised from ‘Hayward' kiwifruit were used as explants to establish regeneration system.The results indicated that the maximum adventitious buds regeneration frequency achieved 80.95% on the mediu of MS+3.0 mg·L-1 6-BA+0.2 mg·L-1 NAA; The most suitable medium for shoot multiplication was MS+3.0 mg·L-1 6-BA+0.2 mg·L-1 NAA+0.1 mg·L-1 GA3 and the three generations average multiplication coefficient was up to 6.38; 1/2MS media with 0.7 mg·L-1 IBA was the most suitable for the rooting of regenerated shoots,and the rooting rate was 100%.The survival rate of hardened regenerated plantlets was as high as 92.38% after transplanting.The results laid a sound foundation for genetic transformation by means of leaf disc method.
‘Hayward' kiwifruit (Actinidia deliciosa)leafhigh frequency regeneration