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‘海沃德’猕猴桃叶片高频直接再生体系的建立

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以‘海沃德’猕猴桃组培苗叶片为外植体,建立叶片直接再生体系.结果表明,叶片再生不定芽最佳培养基为MS+3.0mg·L-1 6-BA+0.1 mg·L-1 NAA,出芽率达80.95%;不定芽增殖最佳培养基为MS+3.0 mg·L-1 6-BA+0.2 mg·L-1 NAA+0.1 mg·L-1GA3,1~3代平均增殖系数达6.38;最适不定芽生根培养基为1/2MS+0.7 mg·L-1 IBA,生根率为100%;试管苗移栽成活率为92.38%.试验结果为采用叶盘法进行‘海沃德’猕猴桃的遗传转化奠定了良好基础.
Establishment of High Frequency and Direct Regeneration System from Leaf of ‘Hayward' Kiwifruit [Actinidia deliciosa (A.Chev.) C.F.Liang et A.R.Ferguson]
Leaves excised from ‘Hayward' kiwifruit were used as explants to establish regeneration system.The results indicated that the maximum adventitious buds regeneration frequency achieved 80.95% on the mediu of MS+3.0 mg·L-1 6-BA+0.2 mg·L-1 NAA; The most suitable medium for shoot multiplication was MS+3.0 mg·L-1 6-BA+0.2 mg·L-1 NAA+0.1 mg·L-1 GA3 and the three generations average multiplication coefficient was up to 6.38; 1/2MS media with 0.7 mg·L-1 IBA was the most suitable for the rooting of regenerated shoots,and the rooting rate was 100%.The survival rate of hardened regenerated plantlets was as high as 92.38% after transplanting.The results laid a sound foundation for genetic transformation by means of leaf disc method.

‘Hayward' kiwifruit (Actinidia deliciosa)leafhigh frequency regeneration

吴秀华、张艳玲、周月、罗克明、汤绍虎

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西南大学三峡库区生态环境教育部重点实验室,生命科学学院,重庆400715

‘海沃德’猕猴桃 叶片 高频再生体系

国家自然科学基金

30871576

2013

植物生理学报
中国植物生理学会,中国科学院上海生命科学研究院植物生理生态研究所

植物生理学报

CSTPCDCSCD北大核心
影响因子:1.532
ISSN:2095-1108
年,卷(期):2013.49(8)
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