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光周期诱导甘蔗花芽分化及成花逆转过程中生理代谢的变化

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以两个在自然条件下难开花的甘蔗品种‘ROC16’和‘ROC22’为材料,研究经光周期诱导甘蔗花芽分化和成花逆转过程中叶片碳水化合物和可溶性蛋白质含量的变化。结果表明,未分化前,两品种经光周期诱导后叶片可溶性总糖、淀粉和蛋白质含量与对照相比无明显变化;从花芽分化诱变期开始,两品种叶片可溶性总糖、淀粉和蛋白质含量迅速增加,明显高于对照(未分化)。随着花芽进一步分化,其叶片可溶性总糖、淀粉含量逐渐降低并低于对照,处在一个较低的水平,蛋白质含量始终高于对照。成花逆转植株在花芽分化前期叶片可溶性总糖、淀粉和蛋白质含量也高于对照,但低于正常抽穗开花植株;之后随着花芽分化的停止,其叶片可溶性总糖、淀粉含量一直保持较稳定的水平,仅蛋白质略有降低。说明甘蔗花芽分化期间大量的基因表达并且合成各种蛋白质的过程需要消耗大量的碳水化合物。
Changes of Sugarcane Flower Bud Differentiation Induced by Photoperiod and Physiological Metabolism in the Process of Flowering Reversion
‘ROC16’ and‘ROC22’ which are shy-lfowering under nature conditions were used as experimental materials. Changes of carbohydrate and soluble protein of the leaf induced by photoperiod in the process of sugarcane lfower bud differentiation and lfowering reversion were studied. The results showed that the contents of soluble total sugar, starch and protein were without apparent changes after induced by photoperiod compared with control before differentiation. But from lfowering induced period, the contents of soluble total sugar, starch and protein were increased rapidly, signiifcantly higher than the control. As the further development of lfower bud differentiation, the contents of total soluble sugar and starch reduced gradually, even lower than the control, still at a low level, while the contents of soluble protein were always higher than the control. The contents of soluble total sugar, starch and protein of the leaf lfowering reversion in the early period of lfower bud differenti-ation were all higher than the control, but lower than the normal lfowering plants. As the lfower bud differentia-tion stop, the contents of soluble total sugar and starch still retained stable level, only protein slightly reduced. The experiment showed that the process of gene expression and protein synthesis in the sugarcane lfower bud differentiation period needed to consume a lot of carbohydrates.

photoperiod inducedlfower bud differentiationlfowering reversionphysiological change

常海龙、王勤南、周峰、黄忠兴、金玉峰、胡后祥、符成、吴其卫、吉家乐、张垂明、陈俊吕、黄锦福、刘少谋

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广州甘蔗糖业研究所海南甘蔗育种场,海南三亚572025

光周期诱导 花芽分化 成花逆转 生理变化

资助广东省工业技术研究院生物工程研究所科技基金广东省科技计划项目广东省科技计划项目现代农业产业技术体系建设专项资金

A2013012011B0203010022011B060400018和2012B061800001CARS-20-1-6

2014

植物生理学报
中国植物生理学会,中国科学院上海生命科学研究院植物生理生态研究所

植物生理学报

CSTPCDCSCD北大核心
影响因子:1.532
ISSN:2095-1108
年,卷(期):2014.(7)
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