Advance of SNP Analysis Based on Whole Genome Resequencing in Crop Gene Mapping
In qualitative trait locus (QTL) mapping of important traits and mutant studies, target genes or ifne mapped QTLs are sometimes hard to be obtained with traditional approaches, which are laborious and time-consuming. in the past few years, the discovery of single nucleotide polymorphism (SNP) markers at the genome level is enabled by new techniques based on whole genome resequencing (WGR). Combined with SNP discovery, validation and genotyping, the WGR-based QTL mapping approach has the potential to rapidly dis-cover candidate genes and causative SNPs. By WGR-based domestication study and genome-wide association analysis of a collection of individuals, and SNP genotyping in hybrid progeny, many QTLs or mutation sites were successfully mapped in crops. This article reviewed the current advances in QTLs mapping or causative mutations detection of important traits in crops based on WGR and SNP analysis, the prospect of the approach in gene/QTL mapping was discussed and analyzed.
NETL
NSTL
万方数据
维普
