Establishment of highly efifcient regeneration system of mangosteen (Garcinia mangostana)
This study established the system ofin vitro culture and multiplication of mangosteen (Garcinia mangostana) using explants from germinated seedlings. The results showed that after 6 weeks of culture inocu-lation, high percentage (62.4%) of nodular callus formations were established from aseptically leaf explants on WPM medium with 1.0 mg·L-1 GA3+1.0 mg·L-16-BA +0.5 mg·L-1 TDZ, and 36% globular callus formations were induced on WPM medium with 0.7 mg·L-1 TDZ+0.5 mg·L-1 6-BA. After 8 weeks of culture, the multipli-cation coefifcient of globular embryogenic callus reached 12.54 on WPM medium supplemented with 2.0 mg·L-1 6-BA and 0.5 mg?L-1 NAA. Subsequently, adventitious buds with 20 mm length were transferred to rooting me-dium for rooting culture. And the best medium for rooting was 1/2MS+0.5 mg?L-1 6-BA+0.5 mg?L-1 NAA+0.1 mg?L-1 IBA+1.0% AC, and the rooting rate was 85.2%.
NETL
NSTL
万方数据
维普
