Molecular cloning and expression analysis of flowering-regulating transcription factor gene PsFUL1 in tree peony (Paeonia suffruticosa)
One important flowering-regulating transcription factor gene FRUITFULL (FUL1) homologous gene was obtained from tree peony (Paeonia suffruticosa 'Luoyanghong') by RT-PCR method. Open reading frame (ORF) length of its cDNA was 768 bp, and could encode 255 amino acids. Sequence alignment and structure domain analysis showed that the protein possessed MADS super family, K-box super family structure domains and multi-domains in ARG80. Also, the protein had stable conservative MADS-box and K-box sequences. NCBI blast analysis found that cloned amino acid sequences had high homology at 69% compared with the FUL-like proteins in Vitis vinifera. This gene was therefore designated as PsFUL1 and GenBank accession number was KX621277. Phylogenetic analysis showed that it had close relationship with VvFUL1 in V. vinif-era, belonging to subfamily AP1/FUL in MADS gene families. The results of qRT-PCR showed that PsFUL1 gene displayed significant expression difference in different organs of P. suffruticosa 'Luoyanghong'. High ex-pression levels were observed in the bud and petal, followed by bract, leaf and root. Among the six different flowering stages of different varieties of tree peony (early-flowering 'Yingrihong', mid-flowering 'Luoyang-hong', late-flowering 'Huawang'), PsFUL1 gene expression level had significant difference with different flow-ering stages and blossoms. The results indicated that PsFUL1 gene had an important regulation effect on the flower formation and blossom in tree peony.
tree peony (Paeonia suffruticosa)PsFUL1different flowering stagesgene expression level
NETL
NSTL
万方数据
维普
