Identification of genes encoding transcription factors of apple cold-resistant dwarfing rootstock in response to cold stress
Using RNA-seq, the transcription factors (TFs) in '71-3-150', a cold-resistant apple dwarf rootstock, responding to cold stress (4°C) of 0, 2, 6, 12 and 24 h were identified. In four comparison groups, 0 h vs 2 h, 0 h vs 6 h, 0 h vs 12 h, 0 h vs 24 h, 83, 98, 115 and 136 differentially expressed TF genes were found respective-ly. These total 228 TF genes, belonging to 32 families and comprising 7.25％ of the total TFs of apple, were 32 WRKYs, 30 AP2/ERFs, 28 bHLHs, 21 MYBs, 13 NACs, 12 HSFs, 10 CO-like, 9 MYB_related, 8 TCPs, 6 bZIPs, 6 C2H2, 6 C3Hs, 6 GATAs, 5 G2-like, 4 GRAS, 4 HD-ZIPs, 4 NF-YCs, 4 ARFs, 3 Dofs, 3 Trihelix , 2 GRFs, 2 NF-YBs, 1 B3, 1 DBB, 1 E2F/DP, 1 EIL, 1 MIKC, 1 M-type, 1 SBP, 1 TALE, 1 YABBY, 1 ZF-HD, suggesting lots of TFs were involved in the rootstock responding to cold stress. Among them, 8 new genes without annotations in apple reference genome were identified. Ten differential expression clusters were ob-tained according to the trends of expression levels of all these genes. Various TF families were involved in each expression cluster indicating the complex mechanism of transcriptional regulation of the rootstock in response to cold stress. Based on functional analysis of indentified TF genes, they played the roles of regulating expres-sion of functional genes in short-term cold acclimation and long-term growth and development, realizing the reestablishment of metabolites and redox balance to acquire cold tolerance, and new homeostasis of growth un-der cold stress.
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NSTL
万方数据
维普
