Molecular cloning and expression profile of BnaGPDH gene in Brassica napus
Glycerol-3-phosphate dehydrogenase (GPDH) is a key enzyme in triacylglycerol (TAG) biosynthe-sis. In order to elucidate the expression feature of BnaGPDHs in Brassica napus, three GPDH full-length cod-ing sequences (1395, 1395 and 1389 bp) were obtained by reverse transcription PCR, and designated as BnaGPDH1, BnaGPDH2 and BnaGPDH3, respectively. Bioinformatic analysis shows that they have the char-acteristics of the GPDH family, and amino acid sequences of BnaGPDHs are highly homologous to GPDHs in other plants. Spatio-temporal expression results show that BnaGPDHs are genes of constitutive expression, with the highest expression in flower and the lowest expression in leaf. BnaGPDH expression levels were gen-erally increased in developing seed at 35 days after flowering, at the same time they were significantly de-creased in developing capsule. BnaGPDHs responded to abiotic stresses, indicating they might be concerned with the mechanism of plant stress resistance.
NETL
NSTL
万方数据
维普
