Tissue culture and shoot regeneration from leaf explants of cold-hardy and semi-dwarf apple rootstock ‘54-118’
Cold-hardy and semi-dwarf apple rootstock ‘54-118’ was selected as material,the effects of basal medium composition and plant growth regulators on proliferation and rooting of in vitro shoots were examined.In vitro leaves were selected as explants,the effects of basal medium composition,plant growth regulators and carbon sources on shoot regeneration were investigated.The results showed that the optimal proliferation medium was QL+1 mg·L-16-BA+0.3 mg·L-1 IBA+30 g·L-1 sucrose,multiplication rate was 5.1.The optimal rooting medium was 1/4MS+0.5 mg·L-1 IBA+20 g·L-1 sucrose,rooting rate was 77.9％,average root number was 3.5.The optimal shoot regeneration medium was modified MS (NM)+3 mg·L-1 6-BA+0.3 mg·L-1 IBA+30 g·L-1 D-sorbitol,regeneration frequency reached 85.6％.
apple rootstockproliferationin vitro rootingin vitro leavesshoot regeneration
NETL
NSTL
万方数据
维普
