Effects of different carbon sources on the growth and accumulation of triterpenoids and flavonoids in tufted buds of Tamarix chinensis
Explants from shoots of Tamarix chinensis were cultured in medium containing five kinds of carbon sources (sucrose, fructose, glucose, maltose and starch) and different concentrations of sucrose (10, 20, 30 and 40 g·L-1), and the growth of cluster buds and contents of secondary metabolite products in them under different treatments were analyzed. The results showed that there were significant effects of different carbon sources and different concentrations of sucrose on the growth of the cluster buds and accumulation of secondary products,and the best medium for their growth contained 10 g·L-1 of sucrose. When using sucrose as a carbon source, the contents of flavonoids and triterpenoids were the highest after the culture of 35 d. The addition of 30 g·L-1 sucrose helped to improve the content of flavonoids markedly [up to 16.65 mg·g-1 (DW)], and 10 g·L-1 of sucrose was optimum for the accumulation of betulic acid, betulin and oleanolic acid [2.67, 6.86 and 0.85 mg·g-1 (DW) respectively]. When the concentration of carbon source was 30 g·L-1, the content of total triterpenoids reached the highest level in cluster buds with the addition of glucose, up to 62.43 mg·g-1 (DW), and the second and the lowest contents were with the addition of sucrose and maltose, respectively. This study will lay a foundation for the large-scale production of medicinal active ingredient from T. chinensis by biological fermentation method.
NETL
NSTL
万方数据
维普
