Research progress on DNA methylation induced by plant tissue culture
Plant tissue culture is one of the important methods of crop improvement,breeding and repro-duction.Based on totipotency,tissue culture regenerants from tissues,organs or cells always look similar genotypically and phenotypically.Specific microenvironmental stress imposed to explants could result in some phenotypic variation.Further studies found that these phenotypic variations are closely related to epigenetic modification changes.The research of DNA methylation leading to somatic clonal variation is the most extensive research.This paper mainly reviewed the latest research of DNA methylation changes in plant tissue culture with different approaches focusing on changes of DNA methylation patterns associ-ated with somatic clonal variation at the genomic level.These findings will contribute to promote crop im-provement and produce excellent germplasm with epigenetic breeding strategy.
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