Identification and characterization of the SlNRT2 gene family and cloning and expression analysis of SINRT2 in Solanum lycopersicum
Nitrate transporter 2 is a high-affinity nitrate transporter,which plays important roles in nitrate uptake and transport in plants.In this study,we performed a comprehensive bioinformatic analysis of NRT2 gene family in tomato genome,and detected the tissue expression pattern of SlNRT2 by heat map analysis.Furthermore,we conducted SlNRT2 promoter activity analysis by using the promoter chimeric GUS reporter gene tansform Arabidopsis thaliana.The bioinformatic analysis showed that there were 5 SI-NRT2s detected in the tomato genome,which mainly located in cell membrane,with protein length ranged from 458 to 531 aa,pl value distributed from 9.05 to 9.33 and instability index ranaed from 31.84 to 40.70.Gene structure analysis showed that the number of exons within SlNRT2 genes was 3-4.Chromosome mapping analysis revealed that the 5 SlNRT2s were distributed on 3 chromosomes.The results of phyloge-netic and conserved motif analysis showed that SlNRT2 gene family was divided into Ⅰ and Ⅱ two groups,it contained ten major conserved motifs,and the protein domain of SlNRT2 family is conserved.Heat map results demonstrated that SlNRT2.1and SlNRT2.2 were expressed in all the tissues,while SlNRT2.3-SlN-RT2.5 were specifically expressed in tomato roots.Analysis of cis-acting elements in promoter and histo-chemical staining revealed that SlNRT2.4 promoter drove GUS gene to accumulate specifically in Arabidop-sis roots,while SlNRT2.3 and SlNRT2.5 expressed in both roots and leaves in Arabidopsis thaliana,which was accordance with the results of heat map analysis.These results will provide a basis for further func-tional research of SINRT2 protein,and also lay a foundation for cultivating transgenic tomato with high ni-trogen utilization efficiency.
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