Identification and expression analysis of CAMTA gene family in apple
The apple calmodulin-binding transcription activators(CAMTA)gene family members were iden-tified and bioinformatically analyzed to investigate their expression characteristics under abiotic stress.The results showed that a total of 12 apple CAMTA genes were identified in this paper.Chromosomal lo-calization showed that most of the MdCAMTA gene members were located on chromosomes 10,12 and 16.Physicochemical property analysis revealed that its CDS sequence length ranged from 405 to 4 284 bp,full gene length ranged from 869 to 8 370 bp,molecular weight ranged from 33 480.24 to 353 015.79 Da,amino acid number of encoded protein ranged from 135 to 1 428 aa,and isoelectric point was 4.8 to 5.25,all less than 7.Phylogenetic analysis showed that apple CAMTA genes were classified into three sub-clades.Conservative motif analysis revealed the presence of Motif 1 and Motif 3 in most MdCAMTA genes,and gene structure analysis showed that MdCAMTAT1 and MdCAMTAT7 have similar gene struc-tures.Cis-acting element prediction showed that the apple CAMTA gene family contains phytohormone re-sponse elements,stress response,and light response elements,most of which are light response ele-ments.Tissue expression pattern analysis revealed that MdCAMTA1 gene was expressed in flowers,fruits,leaves,stems,roots,seeds and seedlings.Quantitative fluorescence analysis showed that MdCAMTA genes mostly played a positive regulatory role under low temperature and drought stress conditions.The preliminary identification of apple CAMTA genes may be involved in drought,low temperature and salt stress response mechanisms,which provides a theoretical basis for further studies on the functions of ap-ple CAMTA genes.
applebioinformatics analysislow temperature stressdrought stresssalt stress
NETL
NSTL
万方数据
