苹果CAMTA转录因子家族的鉴定与表达分析
Identification and expression analysis of CAMTA gene family in apple
董媛媛 1任家玄 1车莉莉 1郭丽丽 1成永娟 1毛娟1
作者信息
- 1. 甘肃农业大学园艺学院,兰州 730070
- 折叠
摘要
鉴定苹果钙调蛋白转录激活因子(CAMTA)基因家族成员并对其进行生物信息学分析,探究其在非生物胁迫下的表达特性.结果表明:本文共鉴定了12个苹果CAMTA基因.染色体定位表明,大多数Md-CAMTA基因成员位于10号、12号和16号染色体上.理化性质分析发现,其CDS序列长度在405～4 284 bp间,基因全长在869～8 370 bp间,分子量在33 480.24～353 015.79 Da间,编码蛋白氨基酸数目在135～1 428 aa之间,等电点为4.8～5.25,均小于7.0.系统进化分析表明,苹果CAMTA基因划为3个亚族.保守基序分析表明大多数MdCAMTA基因中存在Motif 1与Motif 3.基因结构分析显示MdCAMTAT1和MdCAMTAT7具有相似的基因结构.顺式作用元件预测结果显示,苹果CAMTA基因家族包含植物激素响应元件、逆境胁迫响应、光响应等元件,其中大多数为光响应元件.组织表达模式分析发现MdCAMTA1基因在花、果实、叶、茎、根、种子和幼苗中都有表达.荧光定量分析表明MdCAMTA基因在低温、干旱胁迫条件下大多起正调控作用.初步鉴定苹果CAMTA基因可能参与干旱、低温和盐胁迫应答机制,此为进一步研究苹果CAMTA基因的功能提供理论依据.
Abstract
The apple calmodulin-binding transcription activators(CAMTA)gene family members were iden-tified and bioinformatically analyzed to investigate their expression characteristics under abiotic stress.The results showed that a total of 12 apple CAMTA genes were identified in this paper.Chromosomal lo-calization showed that most of the MdCAMTA gene members were located on chromosomes 10,12 and 16.Physicochemical property analysis revealed that its CDS sequence length ranged from 405 to 4 284 bp,full gene length ranged from 869 to 8 370 bp,molecular weight ranged from 33 480.24 to 353 015.79 Da,amino acid number of encoded protein ranged from 135 to 1 428 aa,and isoelectric point was 4.8 to 5.25,all less than 7.Phylogenetic analysis showed that apple CAMTA genes were classified into three sub-clades.Conservative motif analysis revealed the presence of Motif 1 and Motif 3 in most MdCAMTA genes,and gene structure analysis showed that MdCAMTAT1 and MdCAMTAT7 have similar gene struc-tures.Cis-acting element prediction showed that the apple CAMTA gene family contains phytohormone re-sponse elements,stress response,and light response elements,most of which are light response ele-ments.Tissue expression pattern analysis revealed that MdCAMTA1 gene was expressed in flowers,fruits,leaves,stems,roots,seeds and seedlings.Quantitative fluorescence analysis showed that MdCAMTA genes mostly played a positive regulatory role under low temperature and drought stress conditions.The preliminary identification of apple CAMTA genes may be involved in drought,low temperature and salt stress response mechanisms,which provides a theoretical basis for further studies on the functions of ap-ple CAMTA genes.
关键词
苹果/生物信息学分析/低温胁迫/干旱胁迫/盐胁迫Key words
apple/bioinformatics analysis/low temperature stress/drought stress/salt stress引用本文复制引用
基金项目
国家重点研发计划(2022YFD1602106)
甘肃省重大科技专项(22ZD6NA045)
出版年
2024
NETL
NSTL
万方数据