珍稀观赏竹白纹椎谷笹的组培快繁与叶色变异
Tissue culture and leaf color variation of rare ornamental bamboo Sasaella glabra f.albostriata
王婧雅 1王晓芹 1姜可以 1李朝娜 1杨海芸1
作者信息
- 1. 浙江农林大学,省部共建亚热带森林培育国家重点实验室,竹业科学与技术教育部重点实验室,杭州 311300
- 折叠
摘要
白纹椎谷笹(Sasaella glabra f.albostriata)是一种引自日本的珍稀地被观赏竹,为了建立其高效快繁体系,以当年生幼嫩带芽茎段为外植体,对芽增殖培养基、生根培养基及继代培养时间进行筛选.在组培苗移栽1年后进行埋鞭繁殖,研究组培竹苗的繁育能力.结果表明:最佳芽增殖培养基为MS+4.0 mg·L-16-BA+0.001 mg·L-1TDZ,培养30 d时,增殖系数为6.5;最佳生根培养基为MS+0.3 mg·L-1 IBA或0.3 mg·L-1 NAA,生根率可达100％,根系良好,移栽后成活率100％.来源组培苗的微型鞭段埋鞭繁殖1年后,出笋系数为相同规格容器苗微型鞭段的4.21倍.组培苗在移栽第2年出现叶色分离现象,由原来的白绿相间的单一颜色,转变为少量全绿叶、全白叶、白叶绿条纹、绿叶白条纹4种类型的叶片.叶片的光合色素含量均随着叶片绿色条纹加宽而显著增加,但是叶绿素a/b却无显著差异.选取了15个叶色变异相关基因进行了半定量RT-PCR分析,发现psaA、psbB、psbC和psbD基因在叶片的白色组织中转录水平明显低于绿色组织.
Abstract
In this study,buds from the current year young stems were used as explants to establish an effi-cient micropropagation system of Sasaella glabra f.albostriata from Japan including the optimal bud pro-liferation medium,rooting medium and subculture time.One year after transplanting,the micro-rhizome of banboo seedlings were used for propagation to study the breeding ability of tissue cultured bamboo seed-lings.The optimal bud proliferation medium was MS+4.0 mg·L-16-BA+0.001 mg·L-1TDZ,and the prolifera-tion coefficient was 6.5 when culturing for 30 days.The optimal rooting medium was MS+0.3 mg·L-1 IBA or 0.3 mg·L-1 NAA,the roots were strong and the rooting rate was 100％.The survival rate of transplanting was 100％.After planting for one year,the shoot propagation coefficient by the micro-rhizome from tissue cultured seedlings was 4.21 times that of the micro-rhizome from container seedlings with the same size.Leaf color separation of tissue culture seedlings appeared in the second year of transplanting,which changed from the original single color of white-green stripe leaves to a few green leaves,white leaves,white leaves with green stripes,and green leaves with white stripes.The photosynthetic pigment contents of leaves increased significantly with the widening of green stripes,but chlorophyll A/B had no significant difference.A total of 15 genes related to leaf color variation were selected for semi-quantitative RT-PCR analysis,and it was found that the transcription levels of psaA,psbB,psbC and psbD genes in white tissue were significantly lower than those in green tissue.
关键词
白纹椎谷笹/组培快繁/微鞭繁殖/叶色变异Key words
Sasaella glabra f.albostriata/tissue culture/micro-rhizome propagation/leaf color variation引用本文复制引用
基金项目
国家自然科学基金(31901370)
宜宾市引进高层次人才项目(2018YG02)
宜宾竹产业科技专项(2020YLZ004)
安吉林业财政项目(H20210198)
出版年
2024
NETL
NSTL
万方数据