Tissue culture and leaf color variation of rare ornamental bamboo Sasaella glabra f.albostriata
In this study,buds from the current year young stems were used as explants to establish an effi-cient micropropagation system of Sasaella glabra f.albostriata from Japan including the optimal bud pro-liferation medium,rooting medium and subculture time.One year after transplanting,the micro-rhizome of banboo seedlings were used for propagation to study the breeding ability of tissue cultured bamboo seed-lings.The optimal bud proliferation medium was MS+4.0 mg·L-16-BA+0.001 mg·L-1TDZ,and the prolifera-tion coefficient was 6.5 when culturing for 30 days.The optimal rooting medium was MS+0.3 mg·L-1 IBA or 0.3 mg·L-1 NAA,the roots were strong and the rooting rate was 100％.The survival rate of transplanting was 100％.After planting for one year,the shoot propagation coefficient by the micro-rhizome from tissue cultured seedlings was 4.21 times that of the micro-rhizome from container seedlings with the same size.Leaf color separation of tissue culture seedlings appeared in the second year of transplanting,which changed from the original single color of white-green stripe leaves to a few green leaves,white leaves,white leaves with green stripes,and green leaves with white stripes.The photosynthetic pigment contents of leaves increased significantly with the widening of green stripes,but chlorophyll A/B had no significant difference.A total of 15 genes related to leaf color variation were selected for semi-quantitative RT-PCR analysis,and it was found that the transcription levels of psaA,psbB,psbC and psbD genes in white tissue were significantly lower than those in green tissue.
Sasaella glabra f.albostriatatissue culturemicro-rhizome propagationleaf color variation
NETL
NSTL
万方数据
