Response of root system and mhp-miR164a of Malus hupehensis var.pingyiensis to root zone pressure
In this study,Malus hupehensis var.pingyiensis seedlings and transgenic mhp-miR164a Arabidop-sis thaliana were used as materials,the changes of root system and the role of mhp-miR164a were ana-lyzed under the sand culture conditions.The results showed that root zone pressure significantly changed root morphology and structure of M.hupehensis var.pingyiensis and A.thaliana.Root zone pressure down-regulated the expression of mhp-miR164a in the root system of M.hupehensis var.pingyiensis.The pressure of 54-90 kPa significantly inhibited the main roots growth and promoted the lateral roots forma-tion.Under non-pressurized condition,the number of lateral roots of A.thaliana overexpressing mhp-mi-R1 64a was significantly lower than that of wild type.Under the pressure of 54 kPa,the number of lateral roots of wild type A.thaliana increased significantly,while the axial root length and lateral root number of A.thaliana overexpressing mhp-miR164a were significantly lower than those of control,and significantly lower than those of wild type under pressure.The penetration ability of A.thaliana to high hardness medium was significantly decreased by overexpressing mhp-miR164a.These results suggest that mhp-miR164a nega-tively regulates lateral rooting and root resistance to root zone pressure in M.hupehensis var.pingyiensis.
Malus hupehensis var.pingyiensisrootmhp-miR164aroot zone pressure
万方数据
维普
