Structural characteristics and phylogenetic analysis of chloroplast genomes of Apium graveolens and Apium leptophyllum
Both Apium graveolens and Apium leptophyllum are herbaceous plants belonging to the Apiaceae family,known for their abundant nutrients and significant medicinal properties.The chloroplast genomes of A.graveolens and A.leptophyllum were sequenced and analyzed using the Illumina NovaSeq 6000 and Nanopore sequencing platforms,respectively.Comparative analysis of their characteristic differences with other Apiaceae species was conducted.The findings reveal that the total length of the chloroplast ge-nomes of A.graveolens and A.leptophyllum is 152 025 and 158 702 bp,respectively.These genomes contain 87 and 94 protein-coding genes,respectively,and both have 8 rRNA genes and 37 tRNA genes.Although the types and distribution patterns of repeats in A.graveolens and A.leptophyllum are similar,there is a slight dif-ference in the number of repeats.A.graveolens exhibits 202 SSRs,whereas A.leptophyllum has 219 SSRs in its chloroplast genome.Both A.graveolens and A.leptophyllum,like most angiosperms,have codon prefer-ences with A/T at the end.Nucleic acid diversity analysis indicates that the rpl32 gene in the SSC region of the chloroplast genome of these two species could be employes as a potential molecular marker for distin-guishing Apiaceae species.The results of comparative genomic analysis exhibit a high degree of conserva-tion in terms of whole genome size,structure,gene composition,and arrangement among Apiaceae species.No significant gene rearrangements are detected in Apiaceae species,indicating a strong collinearity rela-tionship among them.A phylogenetic tree is constructed based on the chloroplast genomes of 20 Apiaceae species,which demonstrats the high similarity between the A.graveolens and A.leptophyllum and their rela-tionship with other species.This study provides comprehensive information on the chloroplast genomes of A.graveolens and A.leptophyllum,which will contribute to a more comprehensive understanding of the char-acteristics and evolution of chloroplast genomes in Apiaceae plants in the future.
万方数据
维普
