成花转变是显花植物从营养生长到生殖生长的重要发育转变,需要精确地调控使植物最大程度地生产出更多的后代.植物非特异性脂质转移蛋白(non-specific lipid transfer proteins,nsLTPs)参与植物逆境胁迫以及植物生长发育的调控.为研究AtLTP10(lipid transfer protein 10)在拟南芥(Arabidopsis thali-ana)开花调控中的作用,对其蛋白质序列结构、表达模式、亚细胞定位及其功能进行了分析.结果表明,AtLTP10编码一个116 aa的蛋白质,包含一个22 aa的信号肽,亚细胞定位在保卫细胞中.AtLTP10在所有检测的组织中均有表达,在角果中表达量最高,花和根中表达量次之,茎生叶、莲座叶和成熟的种子中的表达水平较低,茎中表达量最低.过表达AtLTP10导致拟南芥延迟开花,而atltp10表现为早花.这些试验结果表明AtLTP10在拟南芥开花调控中起负调控作用.
Lipid transfer protein AtLTP10 involves in flowering regulation
Flowering transition is an important developmental switch from vegetative growth to repro-ductive growth in angiosperms,which needs to be precisely regulated to maximize the production of offspring.Non-specific lipid transfer proteins(nsLTPs)participate in plant abiotic stress and regula-tion of plant growth and development.To study the role of AtLTP10 in flowering regulation in Arabi-dopsis thaliana,this study analyzed its protein structure,expression pattern,subcellular localization and function.The results show that AtLTP10 encodes a protein consisting of 116 amino acids(aa),including a 22 aa signal peptide,and is localized in guard cell.AtLTP10 is expressed in all detected tissues,with the highest expression in siliques,followed by in flower and root,lower expression in stem leaves,rosette leaves and mature seeds,and the lowest expression in stem.Overexpression of AtLTP10 resulted in delaying flowering,while atltp10 displayed early flowering.These results indicate that AtLTP10 plays a negative regulatory role in the flowering pathway of A.thaliana.
万方数据
维普
