Identification and screening of key metabolites and hormones during Chinese jujube embryo abortion by UPLC-MS/MS
To reveal the changes of hormones and metabolomics during jujube(Ziziphus jujuba)embryo development and elucidate the physiological mechanism of jujube embryo abortion,the young embryos of low abortion rate jujube variety'Huizao'(H)and high abortion rate jujube variety'Kongfusucui'(K)were used as experimental materials.Ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)was used to analyze the differences in metabolites and hormone components and con-tents of embryos at different developmental stages of the two materials.A total of 744 metabolites were identified from the different development stages of embryos of H and K varieties,and 597 differential me-tabolites were identified.A total of 283 differential metabolites were identified in the embryos of H and K at the peak period of embryo abortion,which were mainly enriched in tryptophan metabolism(Ko00380),carotenoid biosynthesis(Ko00906),plant hormone signal transduction(Ko04075),zeatin biosynthesis(Ko00908),diterpenoid biosynthesis(Ko00904).During the development of jujube embryos,27 related hormones were identified.There were significant correlations between embryo abortion and 9 hormones including methyl indole-3-acetate(ME-IAA),indole-3-acetic acid(IAA),1-O-indol-3-ylacetylglucose(IAA-Glc),N6-isopentenyladenosine(IPR),trans-zeatin riboside(tZR),dihydrozeatin-7-glucoside(DH27G),trans-zeatin(tZ),gibberellin A15(GA15)and abscisic acid(ABA).The serious abortion of jujube embryo may be closely related to the content of IPR,ABA,DHZ7G,GA15,ME-IAA,as well as the synthesis of phenolic acids(pmb3055,Zmhn002422,Lmtn003441),lipids(Zmgn005057)and flavonoids(mws0789).In addition,the related hormones and metabolites are mainly concentrated in the metabolic pathway of Ko04075,which provides a scientific basis for the targeted transformation of metabolic pathways and hormone regulation for the purpose of reducing the abortion rate of jujube embryo in the future.
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维普
