Mechanism of hydrogen sulfide regulating metabolism of phenolic compounds to alleviate salt stress in tomato
Salt stress is one of the important environmental barriers,which limits crop yield formation.The to-mato was used as the experimental material,and the effects of different concentrations(50,100,200 and 500μmol-L-1)of exogenous hydrogen sulfide(H2S)donor sodium bisulfide(NaHS)on the growth and physiological characteristics of tomato under salt stress were investigated.As well as the metabolism of phenolic com-pounds and its relationship with salt tolerance were investigated.The results showed that the appropriate con-centration of H2S treatment significantly increased the plant height,stem diameter,aboveground fresh and dry weight,root fresh weight and root length,as well as chlorophyll and carotenoid contents of tomato under salt stress,which significantly alleviating the inhibition of tomato growth by salt stress.Exogenous H2S significantly reduced the accumulation of reactive oxygen species(ROS)and malondialdehyde(MDA)content in tomato un-der salt stress,which was closely related to higher activities of SOD,POD,CAT,APX,and the accumulation of osmoregulatory substances such as soluble protein,soluble sugar and proline.In addition,exogenous H2S sig-nificantly increased the content of total phenols,flavonoids,anthocyanidins and antioxidant activities;however,exogenous polyphenol synthesis inhibitor AIP treatment significantly decreased the accumulation of phenolic compounds and significantly reduced the alleviating effect of H2S on oxidative damage in tomato under salt stress.This study elucidated the mechanism of H2S to enhance the effect of salt stress tolerance in tomato and confirmed the role of H2S-induced phenolic compound accumulation of salt stress tolerance in tomato,which provide new ideas for the selection and genetic improvement of salt-tolerant tomato varieties.
tomatosalt stresshydrogen sulfidephenolic compoundsantioxidant system
万方数据
维普
