ADP-Ribosylation Factors play important roles in plant growth and development.The PvARL8 was cloned that a member of the PvARFs family,from the ethanol energy model plant switchgrass.PvARL8 encodes 184 amino acids,which the homology is 97.83%with rice OsARL1b.Expression pattern results suggested PvARL8 is highly expressed in switchgrass elongation stage E3,while expression levels in leaf,stem,flower,and tiller are all higher than root.Moreover,overexpression PvARL8 transgenic plants shown that wider leaves,stems,and more tillers compared to the control.Meanwhile,SLs phytohormones biosynthesis and signaling transduction genes of PvD27,PvD3,and PvD14 relative expression lower than control.All these results indicate that PvARL8 functions potentially in switchgrass plant growth and was proved is the first time.