Analysis of differentially expressed genes in rice seedlings under far-red light irradiation based on transcriptome sequencing
In order to explore the differences of rice seedling growth phenotypes and their responses at the gene under intermittent far-red light irradiation,we designed the experiments of 5,10 and 15 min intermit-tent far-red light irradiation group and a control group without far-red light.After 18 days of incubation,the samples were taken for transcriptome gene sequencing.The analysis of differentially expressed genes took no far-red light as the control group(CK),and intermittent irradiation group with the most significant difference in growth phenotype as the experimental group(T).The results showed that the germination rate of rice seeds could be increased by up to 30％after intermittent irradiation with far-red light.868 dif-ferentially expressed genes were obtained by transcriptome sequencing.Among them,44 significantly up-regulated genes were screened,and functional annotation was enriched in plant hormone signal trans-duction and metabolism,carbohydrate transport and metabolism pathways.119 genes were significantly down regulated,and their functional annotation was enriched in the biosynthetic metabolic pathway of secondary metabolites,diterpenoids and phenylpropanes.Analysis of key differentially expressed genes showed that intermittent far-red light irradiation significantly enhanced gene expression of plant hormone metabolism pathway.The synthesis of gibberellin,brassinolide and auxin increased.Therefore,plant cells elongate and rice rhizome growth is significantly promoted.Analysis of key differentially expressed genes showed that intermittent far-red light irradiation significantly enhanced gene expression of plant hormone metabolism pathway.The synthesis of gibberellin,brassinolide and auxin increased.Therefore,plant cells elongate and rice rhizome growth is significantly promoted.
万方数据
维普
