Screening and analysis of EuFPS1 interacting proteins in Eucommia ulmoides
Farnesyl diphosphate synthase(FPS)is a crucial enzyme in the biosynthetic pathway of Eucom-mia ulmoides rubber.Previous studies have indicated that the EuFPS1 gene facilitates the accumulation of E.ulmoides rubber,however,the specific molecular mechanism of its regulation remains unclear.In this study,a yeast cDNA expression library of E.ulmoides was constructed using SMART homologous recom-bination technology,and the interacting protein was screened using EuFPS1 gene as bait.Through se-quencing and BLAST comparison,41 interacting proteins were initially identified.GO and KEGG analysis showed that these intercalating proteins were mainly involved in regulating biological processes such as porphyrin compound metabolism,UV and cold response,leaf development,as well as metabolic path-ways such as MEP metabolism,porphyrin metabolism,ubiquitination.Three interacting proteins(EuUFM1,EuLHC,EuKS1)were selected to further validate the interactions.qRT-PCR analysis demonstrated that Eu-FPS1,EuUFM1,EuLHC1,and EuKS1 genes were expressed in different tissues of E.ulmoides,with a nega-tive correlation between the expression of EuFPS1 and EuKS1.This study serves as a foundation for the analysis of EuFPS1 and its interaction mechanisms with proteins in E.ulmoides.
万方数据
维普
