Comparative transcriptomics study on differences of alkaloid content in Aconitum carmichaelii roots
In order to understand the reasons for the differences of main alkaloid content in A.carmichaelii from different provenances at the transcriptome level,the principal roots of A.carmichaelii from Beichuan(BM),Qingchuan(QM)and Nanzheng(NM)were selected for non-parametric transcriptome sequencing.The reads gave 128 015 unigenes with an average length of 726 bp and GC％greater than 44.61％.52 496(41.01％),41 296(32.26％),43 561(34.03％)and 34 145(26.67％)unigenes were annotated in Nr,Swiss-Prot,KEGG and KOG,respectively.1 540,1 289 and 1 828 differentially expressed genes(DEGs)were de-tected respectively in BM-vs-NM,BM-vs-QM and NM-vs-QM comparison groups,and these DEGs were en-riched in metabolic pathways,biosynthesis of secondary metabolites,and protein processing in endoplasmic reticulum.Further analysis of the correlation between the DEGs and the content differences of main alkaloids in A.carmichaelii roots showed that,the DEGs related to the content differences of the main alkaloids in the principal roots of A carmichaelii from different provenances were mainly enriched in the metabolic pathways such as flavonoid biosynthesis,MAPK signaling pathway,isoquinoline alkaloid biosynthesis.In this study,candidate genes encoding 13 key enzymes and 17 transcription factors were preliminarily screened,which may be related to the content of diterpenoid alkaloids in A.carmichaelii.The results of the study can provide scientific reference for excellent germplasm selection and molecular breeding of A.carmichaelii.
NETL
NSTL
万方数据
