Multi-transcriptome integration analysis and co-expression network construction of barley under drought stress
Drought stress is one of the important environmental conditions affecting the growth and development of crops,which can lead to reduced yield,quality and even death.As an important wheat crop,barley has better drought resistance than rice and wheat and other cereals,and is considered to be an important model system for analyzing crop drought resistance,this study integrated 138 samples of major barley varieties contained in the six drought stress RNA-Seq datasets of SRA database as analysis data,and analyzed the transcriptome data of drought stress samples(mainly leaves and root tissues)of major barley varieties,and obtained 14709 differential genes.DESeq2 was used for differential gene analysis and enrichment analysis to obtain the transcriptional expression profile of large samples of barley in response to drought stress.The gene co-expression network of barley in response to drought stress was constructed using WGCNA integrated gene expression data,and the tan,greenyellow module and mid-nightblue module significantly related to the response to drought stress in barley were screened from 21 modules,and the core genes in response to drought stress were 36,59 and 23,respectively.The top 10 core genes of each module were searched for Arabidopsis homologous genes,and it was found that such as HORVU.MOREX.r3.6HG0631820(NAC),HORVU.The core genes such as MOREX.r3.7HG0695480(WRKY)have been shown to play an important role in the study of drought stress in other species,suggesting that these core genes may be key candidate genes in re-sponse to drought stress in barley.The co-expression network of barley under drought stress constructed in this study can provide reliable data reference and candidate gene resources for the improvement of drought tolerance in barley.
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万方数据
