Selection of qRT-PCR internal reference gene for succulent plants of Aeonium series
The Aeonium series succulent plants belong to the sedum family,with multiple colored leaf vari-eties and high ornamental value.However,there are few studies on the molecular biology of such plants at home and abroad.In order to screen out the internal reference genes that are stably expressed among dif-ferent cultivated varieties of Aeonium series succulent plants,the leaves of five cultivated varieties of the Aeonium series were used as materials,and eight candidate qRT-PCR(quantitative RT-PCR)reference genes were selected based on the transcriptome libraries of the cultivated varieties Aeonium arboreum'Zwartkop'and Aeonium spp..The expression abundance of these internal reference genes among differ-ent cultivated varieties was analyzed by qRT-PCR.After Genorm,normFinder,BestKeeper analysis and comprehensive analysis,the most suitable internal reference gene GAPDH among different varieties of the Aeonium series succulent plants was finally screened.Using this gene as an internal reference gene,the differentially expressed genes of anthocyanin synthesis in the transcriptome libraries of Aeonium arboreum'Zwartkop'and Aeonium spp.were verified.The results showed that except for AaCHS,the other six genes are indeed differentially expressed in the above two varieties,indicating that GAPDH is a suitable internal reference gene for expression analysis among different cultivated varieties of Aeonium series succulent plants.This study can lay a solid foundation for gene expression analysis among different varieties of Aeonium series succulent plants.
Aeonium series succulent plantsinternal reference genequantitative RT-PCR
NETL
NSTL
万方数据
