Identification of GASA gene family and their expression pattern in response to abiotic stress in foxtail millet
Foxtail millet(Setaria italica),one of the main cereals grown in China,is renowned for its strong stress resistance property,yet the underlying mechanism remains unclear.The GASA(gibberellic acid-stimulated Arabidopsis)family of small polypeptides,ubiquitous across the plant kingdom,plays a pivotal role in a plethora of physiological processes,encompassing plant growth,development,and stress re-sponse.Exploring the structure and function of GASA may shed light on the mechanisms conferring pro-nounced stress resilience in foxtail millet and inform crop improvement strategies.In this study,the GASA gene family was identified in foxtail millet.The sequence,structure,evolutionary status and expression pattern of SiGASAs were analyzed using bioinformatic methods.Meanwhile,RT-qPCR(real time quantita-tive PCR)was used to detect the expression profiles of the SiGASAs under abiotic stress conditions.The findings revealed a repertoire of 12 GASA genes scattered across 6 scaffolds within foxtail millet.Promot-er region of SiGASAs contains cis-acting elements responsive to light,stress and a spectrum of hormones.The encoded proteins were characterized as alkaline,hydrophobic exoproteins,each harboring the con-served GASA domain.The tissue-specific expression patterns varied among SiGASA gene family mem-bers,with generally low expression observed in bud and stem.Notably,SiGASA1-9 were implicated in the plant's response to drought and salinity stress.The insights gleaned from this paper furnish a theoretical reference for the further study of biological function of GASA and its potential application in genetic breed-ing of foxtail millet.
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