Regulating effect of exogenous phenylalanine on the growth of main primary tips in rice seedlings
In this experiment,rice seedlings were treated with exogenous phenylalanine(Phe)and 2-amino-indan-2-phosphonic acid(AIP),a specific inhibitor of phenylalanine ammonialyase(PAL).By comparing the growth indexes of primary roots,lignin content,and PAL activity in root tips,OsCAD1 expression level and CAD activity of root tip cells,and the location of root tip vessels among different treatments,to ex-plore the regulatory effect of exogenous Phe on the growth of primary root tips in rice seedlings.All these results confirmed that exogenous Phe inhibited the length of primary roots and root tips of seedlings,with the improvement of the lignin content,OsCAD1 expression level,CAD activity and PAL activity of root tips,while AIP reversed the impact of Phe.In conclusion,this results indicated that the exogenous Phe en-hanced lignin synthesis efficiency by upregulating the expression level of key gene and its enzyme activity in the synthesis of lignin.Thus,it leads to a reduction in the distance between the position of the vessels and the front end of the root tips,inhibiting root tip elongation,and eventually,delaying primary roots growth in rice seedlings.
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