Functional analysis of pectin lyase gene of Fusarium oxysporum f.sp.melonis
The wilt of melon caused by Fusarium oxysporum f.sp.melonis is a disease that seriously af-fects melon production.Pectin lyase is one of the cell wall degrading enzymes.Whether the pectin lyase gene plays a role in the process of F.oxysporum f.sp.melonis infestation is less reported.This study used the principle of homologous recombination to knock out the FopLb,FopLe,FopL1 and FopL2 via Agrobacte-rium tumefaciens-mediated genetic transformation,and the knockout mutants were subjected to experi-ments on phenotypes,pathogenicity,susceptibility to stress factors.Results showed that the mutant strains △FopLb and △FopL1 exhibited smaller colonies without pigment deposition,whose mycelia were sparse and thinner and hardly produced spores,thus significantly reduced pathogenicity.All mutant strains displayed resistance to exogenous salt stress and cellulose utilization capabilities similar to the wild-type strain.The findings suggest that FopLb and FopL1 play crucial roles in nutritional growth,asexual spore formation,and pathogenicity,with the severe growth defects of △FopLb and △FopL1 likely being a major cause of their reduced pathogenicity.
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