Identification of the CCD gene family and the interacting miRNAs and their roles in drought adaptation in potato
Potato is a kind of drought-sensitive crop,and drought severely affects its yield and quality.Our previous work found that the potato carotenoid cleavage dioxygenase(CCD)gene(StCCD)was highly re-sponsive to drought stress.In this study,based on the sequencing results of potato transcriptome and miRNAome during the drought-rehydration-redrought process,the identification,bioinformatics analysis,and expression profiling of the potato CCD gene family and its interacting miRNAs were conducted at the whole-genome level.Co-expression analysis was also performed to investigate the interaction between drought-responsive StCCDs and their interacting miRNAs during the drought-rehydration cycles.The re-sults showed that 17 CCD genes were identified in potato,located on five chromosomes and divided into six subfamilies.These genes encoded polypeptides ranging from 174 to 1 115 amino acid residues and shared conserved motif 5.Expression profiling analysis revealed that the 17 StCCDs were expressed in dif-ferent tissues and organs of potato plants,as well as under various stresses and plant growth regulator treatments,which demonstrated significant differential expression patterns,indicating the functional di-versification of gene members of StCCD family.Among them,StNCED1,StNCED6,and StCCD4 in the StCCD family exhibited rhythmic responses to drought-rehydration cycles,suggesting that the three members are key genes regulating response and adaptation of drought stress in the StCCD family.Combining miR-NAome sequencing data and bioinformatics prediction,777 miRNAs were found to target 17 members of the StCCD gene family.Co-expression analysis showed an overall negative regulatory relationship between the drought-responsive StCCDs and their corresponding miRNAs,indicating that the interaction between these miRNAs and their targeted StCCDs may be involved in the response and adaptation of potato to drought stress.The results of this study contribute to the understanding of the multifunctionality of the St-CCD gene family and the involvement of StCCDs and their corresponding miRNAs in the response and ad-aptation mechanisms of potato to drought stress,and provide candidate genes for subsequent precise molecular breeding of drought-tolerant potatoes.
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