Functional analysis of tomato SlHDT3 gene in regulating drought and salt stress tolerance
Histone deacetylase(HDACs)genes perform as crucial regulators widely participation in the process of plant growth development,ripening and stress response in plants.To revealed the role of toma-to SlHDT3 gene under drought and salt stress tolerance,wild-type tomato seedlings were subjected to abi-otic stress and plant growth regulators treatment and the relative expression level of SlHDT3 were ana-lyzed by qRT-PCR.It was found that SlHDT3 was involved in the response to various abiotic stresses and plant growth regulators treatments.An RNAi vector was constructed and transferred successfully into wild-type tomato for further research on the detailed functions of the histone deacetylation gene SlHDT3.The result suggested that silencing SlHDT3,there was no significant difference in the growth of WT and Sl-HDT3-RNAi under normal conditions.After drought and salt treatment,the survival rate of SlHDT3-RNAi plants was lower,chlorophyll degradation was faster,proline content,CAT activity and ABA concentration were decreased,while MDA content and total histone H3 acetylation level were increased.The results of RNA-seq analysis data showed that varying degrees of up-regulated or down-regulated of stress-related genes were occur in SlHDT3-RNAi plants compared with the WT.The results suggest that SlHDT3 acts as a stress-responsive gene,plays a role in the positive regulation of abiotic stress tolerance,and may be one of the new target genes in the engineering breeding of salt-and drought-tolerant tomato.
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