Identification and expression analysis of GeBP gene family in red raspberry
Glabrous-enbancer binding protein(GeBP)is a unique protein in plants,which plays an important role in plant growth and development,hormone levels and stress.In this study,using'Heritage'red rasp-berries as materials,the GeBP gene family members were identified and their expression patterns were studied by transcriptome sequencing,bioinformatics analysis and qRT-PCR.The results showed that:three members of the GeBP gene family the protein amino acid number ranged from 236-407,the molecular weight ranged from 26.66-42.25 kDa,and the isoelectric point ranged from 4.84-9.52.RuGeBP-1 and Ru-GeBP-2 are unstable proteins,and RuGeBP-3 is stable.All three are hydrophilic proteins,contain most ami-no acids as serine,have no signal peptide,are non-secreted proteins,are localized in the nucleus,are non-transmembrane proteins,contain DUF573 conserved domain,secondary structure prediction showed that the proteins encoded by RuGeBPs were composed of α helix,β corner,random curling and folding ex-tension chains,and the tertiary structure prediction results were consistent with the secondary structure.The analysis of gene structure and conserved motifs showed that RuGeBP gene family had 0-2 introns,and have six important conserved motifs;Phylogenetic analysis showed that red raspberry and strawberry were more closely related.The promoter cis-acting elements indicated that RuGeBP gene family was mainly in-volved in hormone response,defense and stress response,plant growth and development.RuGeBP gene family had tissue-specific expression,which was mainly expressed in early fruit development and roots.Ru-GeBP-1,RuGeBP-3 might be related to the response mechanism of abscisic acidand other hormones in fruit and the growth a of roots,RuGeBP-2 may be involved in root growth and defense mechanisms.
red raspberry(Rubus idaeus)GeBP transcription factoridentificationbioinformatics analysisexpression analysis
NETL
NSTL
万方数据
