NaCl胁迫对魁核桃幼苗生理生化指标及矿质元素分布的影响
Effects of NaCl stress on physiological and biochemical characteristics and compartmental allocation of mineral element of Juglans major seedlings
姬新颖 1唐佳莉 1李敖 1郑旭 1张俊佩1
作者信息
- 1. 中国林业科学研究院林业研究所,林木遗传育种全国重点实验室,国家林业和草原局林木培育重点实验室,北京 100091
- 折叠
摘要
为筛选核桃耐盐砧木提供理论依据,对魁核桃(Juglans major)幼苗进行NaCl胁迫处理,测定处理后不同时间生理生化指标和矿质元素变化.结果表明:随着NaCl浓度的增加,幼苗各部分生物量、叶片相对含水量呈降低趋势,丙二醛含量有所增加;可溶性蛋白含量呈降低趋势,脯氨酸和可溶性糖大量积累;总酚、总黄酮及抗氧化酶活性均较对照有所增加;在促进脱落酸生成的同时抑制了生长素等的合成.盐胁迫下,Na在根部积累比较多,而K则更多的积累在叶片中,叶片和根部的K/Na比均显著降低;叶片和根中的Ca、Mg含量总体保持相对稳定,变化幅度不大,而Fe、Zn和Cu含量总体呈降低趋势.说明盐胁迫下魁核桃幼苗能够通过增加渗透调节物质含量、提高抗氧化酶活性、调节自身激素平衡、维持养分吸收能力等保护机制,缓解盐胁迫带来的伤害.
Abstract
In order to provide a theoretical basis for screening salt-tolerant walnut rootstocks,the seedlings of Juglans major were treated with salt stress,and changes in physiological and biochemical indexes and mineral elements were measured in different time after treatment.The results showed that with the in-crease of NaCl concentration,the biomass of all parts of the seedlings and leaf relative water content tended to decrease,and malondialdehyde content increased;soluble protein content showed a decreasing trend,and proline and soluble sugar accumulated significantly;total phenols,total flavonoids and antioxi-dant enzyme activities increased compared with the control;and inhibited the synthesis of indole-3-acetic acid and others while promoting abscisic acid production.Under salt stress,Na accumulated more in the roots,while K accumulated more in the leaves,and K/Na ratio was significantly reduced in both leaves and roots;Ca and Mg contents in leaves and roots generally remained relatively stable with little variation,while Fe,Zn and Cu contents generally showed a decreasing trend.It indicated that J.major seedlings under salt stress were able to alleviate the damage and inhibition of salt stress on the plant body by increasing the con-tent of osmotic regulation substances,improving the activities of antioxidant enzymes,regulating their own hormone balance,maintaining the ability of nutrient uptake,and other protective mechanisms.
关键词
魁核桃/NaCl胁迫/渗透调节/抗氧化酶活性/离子平衡/内源激素Key words
Juglans major/NaCl stress/osmotic regulation/antioxidant enzyme activity/ion balance/en-dogenous hormone引用本文复制引用
基金项目
国家重点研发计划(2022YFD2200402)
出版年
2024
NETL
NSTL
万方数据